Students of class 12 Biology should refer to MCQ Questions Class 12 Biology Biotechnology and its Application with answers provided here which is an important chapter in Class 12 Biology NCERT textbook. These MCQ for Class 12 Biology with Answers have been prepared based on the latest CBSE and NCERT syllabus and examination guidelines for Class 12 Biology. The following MCQs can help you to practice and get better marks in the upcoming class 12 Biology examination

Chapter 12 Biotechnology and its Application MCQ with Answers Class 12 Biology

MCQ Questions Class 12 Biology Biotechnology and its Application provided below have been prepared by expert teachers of grade 12. These objective questions with solutions are expected to come in the upcoming Standard 12 examinations. Learn the below provided MCQ questions to get better marks in examinations.

Question. The site of production of ADA in the body is
(a) bone marrow
(b) lymphocytes
(c) blood plasma
(d) monocytes

Question. Genes of interest can be selected from a genomic library by using
(a) restriction enzymes
(b) cloning vectors
(c) DNA probes
(d) gene targets

Question. The protein a-1 antitrypsin is used to treat the
(a) cancer
(b) rheumatoid arthritis
(c) Alzheimer’s disease
(d) emphysema

Question. During the processing of ‘proinsulin’ into mature ‘insulin’
(a) A – chain is removed
(b) C – chain is removed
(c) B – chain is removed
(d) No peptide chain is removed 

Question. The genetically-modified (GM) brinjal in India has been developed for
(a) insect-resistance
(b) enhancing shelf life
(c) enhancing mineral content
(d) drought-resistance

Question. Bt toxin kill the larvae of certain insects 
(a) by binding of activated toxin on mid gut epithelial cells, creating pores, leading to swelling and lysis.
(b) by stopping transcription of larval cells.
(c) by altering central dogma taking place in the cells of gut of larva.
(d) by stopping protein synthesis

Question. What is true about Bt toxin?
(a) Bt protein exists as active toxin in the Bacillus.
(b) The activated toxin enters the ovaries of the pest to sterilise it and thus prevent its multiplication.
(c) The concerned Bacillus has antitoxins.
(d) The inactive protoxin gets converted into active form in the insect gut.

Question. Genetic engineering has been successfully used for producing
(a) transgenic mice for testing safety of polio vaccine before use in humans
(b) transgenic models for studying new treatments for certain cardiac diseases
(c) transgenic cow-Rosie which produces high fat milk for making ghee
(d) animals like bulls for farm work as they have super power

Question. The trigger for activation of toxin of Bacillus thuringiensis is 
(a) acidic pH of stomach
(b) high temperature
(c) alkaline pH of gut
(d) mechanical action in the insect gut 

Question. Cry protein is obtained from
(a) Bacillus thuringiensis
(b) Bacillus subtilis
(c) Clostridium welchi
(d) E. coli

Question. Tobacco plants resistant to a nematode have been developed by the introduction of DNA that produced (in the host cells)
(a) both sense and anti-sense RNA.
(b) a particular hormone.
(c) an antifeedant.
(d) a toxic protein.

Question. Bt corn has been made resistant from corn borer disease by introduction of the gene
(a) cryIAb
(b) ampR
(c) cryIIAb
(d) trp

Question. Which one of the following is commonly used in transfer of foreign DNA into crop plants?
(a) Meloidogyne incognita
(b) Agrobacterium tumefaciens
(c) Penicillium expansum
(d) Trichoderma harzianum

Question. Biopiracy means
(a) use of biopatents
(b) thefts of plants and animals
(c) stealing of bioresources
(d) exploitation of bioresources without authentic permission

Question. Bt cotton is not 
(a) a GM plant
(b) insect resistant
(c) a bacterial gene expressing system
(d) resistant to all pesticides

Question. C-peptide of human insulin is 
(a) a part of mature insulin molecule
(b) responsible for formation of disulphide bridges
(c) removed during maturation of proinsulin to insulin
(d) tesponsible for its biological activity.

Question. GEAC stands for
(a) Genome Engineering Action Committee
(b) Ground Environment Action Committee
(c) Genetic Engineering Approval Committee
(d) Genetic and Environment Approval committee

Question. Cry I endotoxins obtained from Bacillus thuringiensis are effective against
(a) nematodes
(b) bollworms
(c) mosquitoes
(d) flies

Question. A probe which is a molecule used to locate homologous sequences in a mixture of DNA or RNA molecules could be:
(a) a ssRNA
(b) a ssDNA
(c) either RNA or DNA
(d) can be ssDNA but not ssRNA

Question. Pathophysiology is the 
(a) study of physiology of pathogen
(b) study of normal physiology of host
(c) study of altered physiology of host
(d) none of the above

Question. C-peptide of human insulin is
(a) a part of mature insulin molecule.
(b) responsible for formation of disulphide bridges.
(c) removed during maturation of pro-insulin to insulin.
(d) responsible for its biological activity. 

Question. In some children, ADA deficiency can be cured by
(a) bone marrow transplantation
(b) enzyme replacement therapy
(c) both (a) and (b)
(d) none of the above

Question. Maximum number of existing transgenic animals is of
(a) fish
(b) mice
(c) cow
(d) pig

Question. Find out the incorrect statement.
(a) Human protein used to treat emphysema is a-1 antitrypsin.
(b) Human insulin is being commercially produced from a transgenic species of Agrobacterium tumefaciens.
(c) Rosie, the first transgenic cow, produced human protein enriched milk.
(d) Cry I Ab endotoxins obtained from Bacillus thuringiensis is effective against corn borers.

Question. Which of the following statement is correct about Bt toxin ?
(a) Bt protein exists as active toxin in the Bacillus.
(b) The activated toxin enters the ovaries of the pest to sterilize it and thus prevent its multiplication.
(c) The concerned Bacillus has antitoxins.
(d) The inactive protoxin gets converted into active form in the insect gut.

Question. Which one of the following statement is correct?
(a) The proteins encoded by the genes cry I Ac and cry II Ab control cotton bollworms.
(b) Protein encoded by cry I Ab controls corn borer.
(c) Both (a) and (b)
(d) Proteins encoded by cryI Ac and cry I Ab control flies.

Question. Golden rice is 
(a) a variety of rice grown along the yellow river in China
(b) long stored rice having yellow colour tint
(c) a transgenic rice having gene for b-carotene
(d) wild variety of rice with yellow coloured grains

Question. A bioreactor refers to
(a) fermentation tank
(b) tank for biochemical reactions
(c) organisms reacting to a stimulus
(d) tank for biochemical waste

Question. Human insulin is being commercially produced from a transgenic species of
(a) Rhizobium
(b) Saccharomyces
(c) Escherichia
(d) Mycobacterium

Question. In RNAi, genes are silenced using 
(a) ssDNA
(b) dsDNA
(c) dsRNA
(d) ssRNA

Question. The first clinical gene therapy was done for the treatment of 
(a) AIDS
(b) Cancer
(c) Cystic fibrosis
(d) SCID (Severe Combined Immuno Deficiency resulting form deficiency of ADA)

Question. ADA is an enzyme which is deficient in a genetic disorder SCID. What is the full form of ADA? 
(a) Adenosine deoxyaminase
(b) Adenosine deaminase
(c) Aspartate deaminase
(d) Arginine deaminase

Question. Which of the following statements is correct?
(a) The current interest in the manipulation of microbes, plants and animal has raised serious ethical issues.
(b) One possible risk of genetic engineering is the accidental production of dangerously resistant microorganisms.
(c) Although risks are possible, genetic engineering appears to offer more of contribution to human welfare than threats.
(d) All of the above

Question. Select the correct statement.
(a) Genetic engineering works only on animals and has not yet been successfully used on plants.
(b) There are no risks associated with DNA technology.
(c) The first step in PCR is heat which is used to separate both the strands of target DNA.
(d) DNA from one organism will not bond to DNA from another animal.

Question. Silencing of mRNA has been used in producing transgenic plants resistant to
(a) bollworms
(b) nematodes
(c) white rusts
(d) bacterial blights

Question. a-1-antitrypsin is
(a) an antacid
(b) an enzyme
(c) used to treat arthritis
(d) used to treat emphysema

Question. Human insulin is being commercially produced from a transgenic species of
(a) Rhizobium
(b) Saccharomyces
(c) Escherichia
(d) Mycobacterium

Question. What is antisense technology?
(a) When a piece of RNA that is complementary in sequence is used to stop expression of a specific gene.
(b) RNA polymerase producing DNA.
(c) A cell displaying a foreign antigen used for synthesis of antigens.
(d) Production of somaclonal variants in tissue cultures.

Question. Silencing of a gene could be achieved through the use of
(a) RNAi only
(b) antisense RNA only
(c) both RNAi and antisense RNA
(d) none of the above

Question. Cry II Ab and cry I AC produce toxins that control
(a) cotton bollworms and corn borer respectively.
(b) cotton borer and cotton bollworms respectively.
(c) tobacco budworms and nematodes respectively.
(d) nematodes and tobacco budworms respectively.

Question. What is the disadvantage of using porcine insulin (from pig) in diabetic patients?
(a) It leads hypercalcemia.
(b) It is expensive.
(c) It may cause allergic reactions.
(d) It can lead to mutation in human genome.

Question. Which technique would to be completely curative in SCID ?
(a) Gene therapy in adult stage.
(b) Gene therapy in embryonic stage.
(c) Bone marrow transplantation.
(d) Enzyme replacement therapy.

Question. In order for gene therapy to be most effective, genes should be inserted in
(a) WBC
(b) RBC
(c) stem cells
(d) all of these

Question. Which of the following Bt crops is being grown in India by the farmers?
(a) Cotton
(b) Brinjal
(c) Soyabean
(d) Maize

Question. Which of these is used as vector in gene therapy for SCID?
(a) Arbovirus
(b) Rotavirus
(c) Retrovirus
(d) Parvovirus

Question. Which of the following is a correct statement?
(a) “Bt” in Bt-cotton indicates that it is genetically modified organism produced through biotechnology.
(b) Somatic hybridization involves fusion of two complete plant cells carrying desired genes.
(c) The anticoagulant hirudin is being produced from transgenic Brassica napus seeds.
(d) “Flavr Savr” variety of tomato has enriched the production of ethylene which improves its taste.

Question. Which one of the following techniques made it possible to genetically engineer living organism ?
(a) Recombinant DNA techniques
(b) X-ray diffraction
(c) Heavier isotope labelling
(d) Hybridization

Question. The genetic defect, adenosine deaminase (ADA) deficiency may be cured permanently by
(a) administering adenosine deaminase through injection
(b) bone marrow transplantation
(c) enzyme replacement therapy
(d) introducing isolated gene from marrow cells producing ADA into the cells at early embryonic stages

Question. Biotechnology deals with industrial scale production of biopharmaceuticals and biological products using genetically modified
(a) microbes only
(b) fungi only
(c) plants and animals only
(d) all of the above

Question. Which of the following statements about transgenic animals is/are false?             
(i) Transgenic animals are designed to study how genes are regulated.
(ii) They are specially made to serve as models for human diseases.
(iii) Transgenic cow Rosie was created to produce the human protein a-1-antitrypsin.
(iv) Transgenic mice are used to test the safety of vaccines.
(a) (iii) only
(b) (i) and (iii) only
(c) (ii) only
(d) (ii) and (iii) only

Question. The genetically-modified (GM) brinjal in India has been developed for       
(a) insect-resistance
(b) enhancing shelf life
(c) enhancing mineral content
(d) drought-resistance.

Question. Protein encoded by gene cryIAb controls the infestation of which of the following insects?   
(a) Cotton bollworm
(b) Anopheles mosquito
(c) Corn borer
(d) Aedes mosquito

Question. Which of the following is known as ‘Flavr Savr’?       
(a) Specific variety of pesticide
(b) Breed of chicken
(c) Transgenic tomato
(d) Toxic insecticidal protein

Question. The illegal and unlawful development of biomaterials without payment to the inhabitants of their origin is called             
(a) biopatent
(b) biotechnology
(c) biowar
(d) biopiracy.

Question. Some of the steps involved in the production of humulin are given below. Choose the correct sequence.             
(i) Synthesis of gene (DNA) for human insulin artificially.
(ii) Culturing recombinant E.coli in bioreactors.
(iii) Purification of humulin.
(iv) Insertion of human insulin gene into plasmid.
(v) Introduction of recombinant plasmid into E.coli.
(vi) Extraction of recombinant gene product from E.coli.
(a) ii, i, iv, iii, v, vi
(b) i, iii, v, vi, ii, iv
(c) i, iv, v, ii, vi, iii
(d) iii, v, ii, i, vi, iv

Question. Basic principle of developing transgenic plants and animals is to introduce the gene of interest into the nucleus of               
(a) somatic cell
(b) vegetative cell
(c) germ cell
(d) body cell.

Question. RNA interference which is employed in making tobacco plant resistant to Meloidogyne incognita is essentially involved in               
(a) preventing the process of replication of DNA
(b) preventing the process of translation of mRNA
(c) preventing the process of splicing of hnRNA
(d) preventing the process of transcription.

Question. What triggers the activation of protoxin to active toxin of Bacillus thuringiensis in boll worm?         
(a) Acidic pH of stomach
(b) Body temperature
(c) Moist surface of midgut
(d) Alkaline pH of gut

Question. Use of bioresources by multinational companies and organisations without authorisation from the concerned country and its people is called           
(a) bio-infringement
(b) biopiracy
(c) biodegradation
(d) bioexploitation.

We hope the above multiple choice questions for Class 12 Biology for Chapter 12 Biotechnology and its Application provided above with answers based on the latest syllabus and examination guidelines issued by CBSE, NCERT and KVS are really useful for you. Biotechnology and its Application is an important chapter in Class 12 as it provides very strong understanding about this topic. Students should go through the answers provided for the MCQs after they have themselves solved the questions. All MCQs have been provided with four options for the students to solve. These questions are really useful for benefit of class 12 students. Please go through these and let us know if you have any feedback in the comments section.